G-418 Sulfate [Geneticin] (IC-1808)

G-418 Sulfate（Geneticin） Antibiotic is an aminoglycoside antibiotic, related to Gentamicin, toxic to bacteria, yeast, protozoans and helminthes, and higher plant and mammalian cells. G-418 Sulfate（Geneticin） Antibiotic, also known as Antibiotic G-418 Sulfate, is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. G-418 Sulfate（Geneticin） Antibiotic is used as a selective agent in molecular genetics experiments. Resistance to G-418 Sulfate（Geneticin） Antibiotic is conferred by the neomycin resistance genes, which are dominant and are located on both transposons Tn601 (903) (aminoglycoside phosphotransferase 3′ [I] or APH [3′]I) and Tn5 (aminoglycoside phosphotransferase 3′[II] or APH [3′]II). Liquid G-418 Sulfate（Geneticin） Antibiotic is supplied as a clear, colorless solution containing 100mg/mL active G-418 Sulfate（Geneticin） Antibiotic.

Usage:Selection in mammalian cells is usually achieved in 3 to 7 days with concentrations ranging from 400 to 1000 μg/ml.Cells that are dividing are affected more rapidly than non-dividing cell.

Specifications

Product concentration: 100 mg/ml

CAS number: 108321-42-2

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Purity: ≥ 95% (HPLC)

Endotoxin level: < 0.5 EU/mg

Physicochemical characterization: pH, appearance

Cell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell lines

Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells

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